Donkey Kong Country 3 is a platformer where both Donkey Kong and Diddy Kong are kidnapped by a new Kremling leader, prompting Dixie and Kiddy Kong to embark on a rescue mission. They're aided by the Brother Bears, thirteen bears that can be befriended with Bear Coins found throughout levels, offering assistance in various ways.
The gameplay revolves around navigating through diverse environments, battling new enemies, and facing off against huge bosses at the end of each world. Along the way, players encounter other members of the Kong family who provide support and assistance when needed. The game also features bonus missions, including a first-person shooter-style party game.