The story follows four brothers, Leonardo, Donatello, Michelangelo, and Raphael, who are trained in ninjutsu by a wise sensei. They must navigate the city of Manhattan to stop an evil villain named Shredder from unleashing a deadly virus that turns people into mutants.
Gameplay revolves around fighting against hordes of mutated creatures and navigating through levels set in iconic New York City locations. Players control each turtle brother, using their unique skills and abilities to take down enemies and solve puzzles.