Ms. Splosion Man is a platformer game where you play as Ms. Splosion Man, a female version of the hero from 'Splosion Man. The story revolves around the evil scientists capturing the original hero and celebrating their victory, but things take a turn when Ms. Splosion Man is created due to a misfortune. After her introduction, the story takes a backseat to humorous comments from the protagonist.
Gameplay involves using Ms. Splosion Man's ability to self-explose strategically up to three times in a row, which allows for a triple jump. Players must use the environment and timing to avoid traps in levels, with some puzzles to solve that involve switches. The game also features leaderboards where players can compete for better times or replay levels to achieve high scores.