Spider: The Video Game follows the story of a scientist whose mind is transferred into a cybernetic spider after a lab break-in. His goal is to locate the intruders who have stolen his research and body, navigating through various environments along the way.
The gameplay revolves around controlling the cybernetic spider as it moves forward, backward, jumps, and climbs walls. The player can also upgrade certain legs with power-ups, such as flamethrowers, to aid in their progress. However, other cybernetic creatures released during the break-in serve as obstacles and hazards that must be overcome.