The game Rogue Trooper begins with Rogue's comrades being brutally massacred by a traitor General, setting off the story. The first mission is launched from a drop-ship where Rogue and his team come under fire from the Norts due to leaked information. The game introduces Rogue's skills and Bio-chips, which provide unique abilities such as Gunnar's sentry function and Helm's ability to access computer consoles.
The gameplay revolves around stealth and strategy as Rogue navigates through levels trying to take down the enemy by surprise or unleash hell if necessary. The player must track down the traitor General while using a variety of arsenal and tactics at their disposal. The game features three skill levels - Normal, Hard, and Massacre - which unlock cheats upon completion.
The PlayStation 2 version is identical across all platforms, allowing users to create multiple profiles and utilize checkpoint saves to minimize progression loss in case of death. The game also supports multiplayer with four playable characters: Rogue, Helm, Gunnar, and Bagman, each with unique attributes. Players can compete in five arenas with different objectives, including defending against waves of Norts or reaching an objective point.