Route 16 Turbo is an action-packed game where players take control of a car, navigating through various routes while collecting valuable items. However, they must also avoid being chased by enemy cars that will stop at nothing to crash into them.
The game features a bird's eye view map of the 16 individual maze rooms, which contain different items and rewards. Players can collect these items to increase their score and progress through the levels. Each level presents a new challenge, with obstacles such as dead-ends, zig-zags, multiple exits, and enemy cars that will give chase.
The player's car has a turbo boost ability, which provides a burst of speed but also increases fuel usage. Running out of fuel will result in losing a life, while any remaining fuel earns the player bonus points. With three difficulty levels to choose from – easy, normal, and difficult – players can test their skills against increasingly challenging opponents.