Biker Mice from Mars is a single-player action game based on the Biker Mice from Mars cartoon. The mice of Mars and the Catatonian empire fight a war on Mars for control of the planet. Either side is searching for a device that can turn the war in their favor. This device, the Regenerator, is located somewhere on Earth. Three mice have been sent to earth to find the device and retrieve it, fighting off the Catatonian under the leadership of General Hairball and Cataclysm, as well as billionaire Ronaldo Rump.
The game offers 15 missions and three playable characters: Throttle, Modo, and Vinnie. There are race and brawler missions. In the race missions, the player rides their bike and can jump and use various items like turbo boosts and weapons. While racing, the player must shoot at enemies standing on the track. In the brawler mode, the player has to beat up enemies in third-person perspective.