The game world features an explorable open-world Manhattan, along with a hub that includes familiar Marvel locations such as the Marvel HQ. Players can also explore self-standing story mode levels set in various worlds, including Asgard, Bifrost, and Asteroid M. Throughout Manhattan, players can interact with Marvel-related buildings, each with its own storyline narrated by Deadpool.
There are over 150 playable characters in the game, each with their unique Super Powers and Super Moves that affect how they interact with the environment. In-game dialogues are voiced by a cast of 27 actors. The game also features a two-player mode where players can take turns using either the GamePad's display or the TV screen to avoid split-screen issues.