Super Monkey Ball: Step & Roll sees AiAi and his friends trapped inside balls once again, with the goal of rolling them to the exit point of various courses. The gameplay involves tilting the Wii Remote to change the angle of the ball, while new features include using the Wii Balance Board for in-game jumps.
The game offers several modes, including single-player, co-operative, and multi-player modes. In co-operative mode, players use the Wii Remote and balance board to navigate levels with varying obstacles. Multi-player mode supports up to four players, utilizing split-screen views. A "Marathon" mode challenges players to complete levels without dying, while minigames allow for competitive play.
Gameplay indicators include a speed meter, number of lives and bananas gathered, current score, and timer. Stages are named and feature bonus stages with increased rewards. The game's scoring system values speed, with the final score determined by time, stage points, and clear points.