Biker Mice from Mars is a single-player action game based on the Biker Mice from Mars cartoon. The Mice of Mars and the Catatonian empire fight a war on Mars for control of the planet. Either side is searching for a device that can turn the war in their favor. This device, known as the Regenerator, is located somewhere on Earth. Three mice have been sent to earth to find the device and retrieve it, fighting against the Catatonian under the leadership of General Hairball and Cataclysm, as well as billionaire Ronaldo Rump.
The game offers 15 missions and three playable characters: Throttle, Modo, and Vinnie. There are two types of missions: race and brawler. In the race missions, the player rides on his bike and can jump and use various items like turbo's and weapons while racing. While racing, the player must shoot at enemies standing on the track. In the brawler mode, the player has to beat up enemies in third-person perspective.